QR-code enabled additive manufactured multiplex-immunosensor to detect DENV serotypes in dengue patient validate with indirect fluorescence antibody test (IFAT)

Abstract

The current work shows the advancement of an additive manufactured multiplex immunosensor for the detection of dengue serotypes 1&2 in dengue patients on a single device with the LOD of 12.5 ng/ml. In this work, we created a QR-Code enabled additive manufactured case comprised of a paper-based electrode coated with ZnO NPs, which helps to enhance the detection signal and make it more selective, both serotype antibodies (DENV1-Ab & DENV2-Ab) were employed against DENV serotypes (DENV1-Ag & DENV2-Ag. QR-code technology was also integrated with the established platform to deliver sensor supporting information so that anybody may quickly obtain supporting sensing result details by scanning with a smartphone. The proposed highly advanced platform successfully detected DENV serotypes in dengue patients and showing a wide range of detection from 12.5 to 200 ng/ml with a LOD of 12.5 ng/ml.The results were also validated with conventional testing, i.e., Indirect Fluorescence Antibody Test (IFAT), so the developed multiplex-sensor became more applicable for the detection of DENV serotypes on a single tool having high sensitivity and selectivity, with a potential of commercialization.