Abstract
This study was performed to compare indirect fluorescence antibody (IFA) test with competitive ELISA (cELISA) for detection of antibodies to Anaplasma marginale in cattle. In this study, a total of 484 cattle were examined on farms that had a positive history of anaplasmosis. Thin blood smears were prepared from each examined cattle and the cELISA and IFA tests were performed. Samples in which the results of microscopic examination and cELISA were compatible were used as a “gold standard” to define sensitivity and specificity of IFA test and these were used to give “real diagnosis”. According to the test result; 62 of 69 samples found positive in “real diagnosis” were positive in IFA test, and all of the 187 samples that were negative in the real diagnosis were defined as negative in IFA test. When compared to gold standard the sensitivity and specificity of the IFA test were 88.95 and 100%, respectively. In conclusion, both IFA and cELISA tests can be used in the diagnosis of A. marginale. Key words: Anaplasma marginale, IFA test, cELISA.
Highlights
Bovine anaplasmosis occurs in tropical and subtropical areas throughout the world and the disease is a major constraint to cattle production in many countries (Kocan et al, 2010)
In order to define positive cut off for the indirect fluorescence antibody (IFA) test, 20 negative control sera were diluted twice with PBS, starting from 1/40. When each of these samples was subjected to IFA test and antigen reaction was analyzed, it was seen that non-specific fluorescence signals were dense, at dilutions of 1/320 and below
Comparison of microscopic examination results according to the competitive ELISA (cELISA) is given at Table 1
Summary
Bovine anaplasmosis occurs in tropical and subtropical areas throughout the world and the disease is a major constraint to cattle production in many countries (Kocan et al, 2010). The species that cause anaplasmosis in cattle are Anaplasma marginale and A. centrale. The severity and death rate increases with the advance in age. Clinical anaplasmosis is more commonly encountered in cattle older than 1 year of age (Jones et al, 1968). Over 2 years old, clinical symptoms may include abortion, icterus, fever and often death in animals (Kocan et al, 2004). The animals that recovered clinically are the life long carriers of the agent and they are porter of the disease (Kocan et al, 2000; Stokka et al, 2000; Tassi et al, 2002)
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