Abstract
BackgroundMaize rough dwarf disease (MRDD), a widespread disease caused by four pathogenic viruses, severely reduces maize yield and grain quality. Resistance against MRDD is a complex trait that controlled by many quantitative trait loci (QTL) and easily influenced by environmental conditions. So far, many studies have reported numbers of resistant QTL, however, only one QTL have been cloned, so it is especially important to map and clone more genes that confer resistance to MRDD.ResultsIn the study, a major quantitative trait locus (QTL) qMrdd2, which confers resistance to MRDD, was identified and fine mapped. qMrdd2, located on chromosome 2, was consistently identified in a 15-Mb interval between the simple sequence repeat (SSR) markers D184 and D1600 by using a recombinant inbred line (RIL) population derived from a cross between resistant (“80007”) and susceptible (“80044”) inbred lines. Using a recombinant-derived progeny test strategy, qMrdd2 was delineated to an interval of 577 kb flanked by markers N31 and N42. We further demonstrated that qMrdd2 is an incompletely dominant resistance locus for MRDD that reduced the disease severity index by 20.4%.ConclusionsA major resistance QTL (qMrdd2) have been identified and successfully refined into 577 kb region. This locus will be valuable for improving maize variety resistance to MRDD via marker-assisted selection (MAS).
Highlights
Maize rough dwarf disease (MRDD), a widespread disease caused by four pathogenic viruses, severely reduces maize yield and grain quality
MRDD is caused by four pathogenic viruses [1], namely, Maize rough dwarf virus (MRDV), Mal de Rio Cuarto virus (MRCV), Rice black-streaked dwarf virus (RBSDV) and Southern rice black-streaked dwarf virus (SRBSDV), which are prevalent in Europe [2], South America [3], Asia [4], and Northern China [5], respectively
These results suggested that the genetic factor plays a major role in maize resistance to MRDD within the recombinant inbred line (RIL) population
Summary
Maize rough dwarf disease (MRDD), a widespread disease caused by four pathogenic viruses, severely reduces maize yield and grain quality. MRDD is caused by four pathogenic viruses [1], namely, Maize rough dwarf virus (MRDV), Mal de Rio Cuarto virus (MRCV), Rice black-streaked dwarf virus (RBSDV) and Southern rice black-streaked dwarf virus (SRBSDV), which are prevalent in Europe [2], South America [3], Asia [4], and Northern China [5], respectively. These pathogenic viruses are naturally transmitted by the small brown planthopper Laodelphax striatellus in a persistent manner [6] and are not spread. Collecting and identifying various resistant germplasms will facilitate the development of resistant maize cultivars, and it is especially important to map and clone resistant genes to MRDD
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