Abstract
Parenchymal cells (hepatocytes) of adult mammalian liver normally contain only the type L isozyme of pyruvate kinase, whereas nonparenchymal cells of liver contain the M2 isozyme. A net shift in favor of type M2 pyruvate kinase has been seen in homogenates of rat liver during the 2 to 3 weeks of regeneration following partial hepatectomy. We have examined the isozyme content of parenchymal cells isolated during this regeneration period and find both type L and type M2 pyruvate kinases. However, we do not find intermediate hybrids of these two isozymes, even though such hybrids have been demonstrated in other tissues and produced in vitro. Hence, our results suggest that hepatocytes are capable of synthesizing type M2 pyruvate kinase during liver regeneration, and that synthesis of the L and M2 isozymes during this period may be mutually exclusive events for a given cell.
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