Abstract
Two bands of pyruvate kinase activity were demonstrated in normal hemolysates with high voltage electrophoresis at pH 5.3 using cellulose acetate strips. Heterozygotes with red cell pyruvate kinase deficiency also exhibited two bands. Six of ten homozygous patients showed two bands, the remaining four only one band. No mutant enzyme with abnormal electrophoretic mobility could be demonstrated among the ten homozygous individuals with pyruvate kinase deficiency. High voltage electrophoresis may be an additional procedure for the characterization of PK variants in patients with congenital hemolytic anemia associated with PK deficiency.
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