Abstract
A modification of Moellering & Gruber's enzymatic‐spectrophotometric method (1966) has been employed for determining the pyruvate and citrate concentrations in rabbit aqueous humour. Aqueous humour samples from non‐fasting, female albino rabbits of Danish country breed, aged from 4 to 36 months, were found to have the following concentrations (mean ± s.d.):pyruvate (28 animals): 339 μmol/1 ± 50citrate (28 animals): 354 μtmol/1 ± 48glucose (9 animals): 6.1 mmol/1 ± 0.6, determined with a Beckmann glucose analyzer.The following concentration ratios were found in rabbit aqueous humour:pyruvate/citrate: 0.98 ± 0.18, glucose/pyruvate: 19.2 ± 2.2, and glucose/citrate: 17.3 ± 2.5.The pyruvate/citrate ratio was found to decrease with increasing age (0.05 > P > 0.02).Citrate and pyruvate determinations in the same watery pool yielded a day‐to‐day accuracy of the method expressed by coefficients of variation of 7.6% for pyruvate and 6.1% for citrate.
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