Abstract

The results are reported of enzymatic‐colorimetric determinations of citrate concentrations in aqueous humour and plasma, and of pyruvate concentrations in aqueous humour from cattle aged from 1 to 10 years. The mean citrate concentration in aqueous humour samples from 32 animals was 86.5 ± 16.3 μmol/1 (mean value ± standard deviation). In plasma samples from 27 animals the mean citrate concentration was 110.3 ± 47.2 μmol/1.In aqueous humour samples from 24 of the 32 cattles, the mean pyruvate concentration was 172.6 ± 35.6 μmol/1.Regression analysis revealed a relationship between age and the citrate concentrations in aqueous humour (p < 0.001) and between age and the pyruvate concentrations in aqueous humour (0.05 > p > 0.02), the citrate concentrations rising and the pyruvate concentrations falling with increasing age. No relationship was demonstrated between the plasma citrate concentrations and age (0.50 > p > 0.40).

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