Pyrogallol-based dipodal optical probe as new smart analytical tool for sustainable detection of cobalt in biosystem

Abstract

The present research focuses on the micro-level detection of cobalt ions in biological and environmental samples using a new probe. The probe is a multifunctional symmetrical dipodal molecule with two pyrogallol binding units attached to the malonate scaffold through a propylene spacer. It was synthesized and characterized by 1H NMR, 13C NMR, IR, electronic spectroscopy, and mass spectrometry. The molecule's binding, thermodynamic, and photophysical properties are also described. The designed probe demonstrates an excellent sensing ability for Co(II) based on the ESIPT “OFF-ON” fluorescence mechanism. The experiments explore the high selectivity of the ligand for cobalt sensing over a wide range of metal ions of biological and environmental importance. The fluorescence intensity shows a linear response to Co(II) in 5–100 μM concentration with a detection limit of 8.75 x 10−5 and a 2.65-fold enhancement in the intensity. These results establish its potential application as a fluorescence sensor. The probe is also employed as a colorimetric sensor for the qualitative determination of cobalt ions in DMSO solution. The interesting behavior of the probe motivated us further to study its coordination properties with divalent cobalt in solution. The pre-organized assembly with an appropriate cavity size favors the ligand for an efficient Co(II) encapsulation by coordinating through imine-Ns and aromatic ring-Os donors, giving high formation constants.