Chemical Constituents of Euphorbia sororia from Xinjiang

Abstract

Euphorbia sororia is a therophyte growing in Central Asia and Hotan in Xinjiang Uyghur Autonomous Region and belongs to Euphorbia cyparissias. As a traditional Uyghur herbal medicine, it is used to treat water retention, to ease tension, to clear the mind, and as an anthelmintic. Clinically, it has been used to treat constipation, urinary frequency, headache caused by hypertension, liver cirrhosis, edema, and scabies inflammation [1, 2]. The leaves of Euphorbia sororia were collected from Hotan Prefecture of Xinjiang Uyghur Autonomous Region of P. R. China in August 2011. In the present study, a series of separation and purification method such as silica gel column chromatography, polyamide column chromatography, and Sephadex LH-20 column chromatography were applied for the separation and purification of compounds from the leaves of Euphorbia sororia. As a result, eight compounds were obtained and identified, and their structures were identified using spectral techniques and by comparison of their spectral data with the literature or authentic standard substances. The eight compounds were isolated from the plant for the first time. In addition, three other compounds, including kaempferol 3-O-D-glucopyranoside, octacosanol, and -sitosterol, isolated from Euphorbia sororia were similar to compounds reported previously [3, 4]. Kaempferol 3-O-D-Glucuronide (1). Yellow powder, mp 186–189 C. ESI-MS m/z 461 [M–H]–. The 1H NMR (400 MHz, DMSO-d6) and 13C NMR (100 MHz, DMSO-d6) spectral data of compound 1 were in accord with the reported values in the literature [5]. Kaempferol 3-O-L-Arabinoside (2). Yellow powder, mp 183–186 C. The 1H NMR (400 MHz, CD3OD) and 13C NMR (100 MHz, CD3OD) spectral data of compound 2 were in accord with the reported values in the literature [6]. Kaempferol 3-O-D-Xylopyranoside (3). Yellow powder, mp 202–205 C. The 1H NMR (400 MHz, DMSO-d6) and 13C NMR (100 MHz, DMSO-d6) spectral data of compound 3 were in accord with the reported values in the literature [7]. Quercetin 3-O-D-Glucoside (4). Yellow powder, mp 188–190 C. ESI-MS m/z 611 [M + H]+, 633 [M + Na]+. The 1H NMR (400 MHz, DMSO-d6) and 13C NMR (100 MHz, DMSO-d6) spectral data of compound 4 were in accord with the reported values in the literature [8]. Isoscopoletin (5). Yellow powder, mp 183–186 C. ESI-MS m/z 193 [M + H]+. The 1H NMR (400 MHz, DMSO-d6) and 13C NMR (100 MHz, DMSO-d6) spectral data of compound 5 were in good agreement with the reported values in the literature [9]. -Amyrin 3-O-D-Glucopyranoside (6). White powder, mp 80–82 C. The 1H NMR (400 MHz, DMSO-d6) and 13C NMR (100 MHz, DMSO-d6) spectral data of compound 6 were in accord with the reported values in the literature [10]. Hexacosanoic Acid (7). White powder, mp 73–75.5 C. The 1H NMR (400 MHz, CDCl3) and 13C NMR (100 MHz, CDCl3) spectral data of compound 7 were in accord with the reported values in the literature [11].