Pyrimido quinolin derivative: A potential inhibitor for pandemic influenza A (H1N1) viral growth and its replication

Abstract

BackgroundTo explore the pyrimido quinolin derivative, 4-methyl pyrimido (5, 4-c) quinoline-2,5(1H, 6H)-dione and its potential antiviral activity against influenza virus. MethodsPyrimido quinolin derivative was synthesized and characterized. Madin–Darby Canine Kidney (MDCK) cells were cultured and antiviral assays were performed to evaluate its antiviral activity against pandemic influenza A/H1N1 (2009) virus. Results and discussionThe effective inhibitory concentration (EC50) of synthesized compound against influenza virus was determined as 21 μM. RT-PCR analysis revealed that, at the 21 μM concentration of test compound found to inhibit the influenza viral RNA synthesis. The western blot study revealed that 21 μM of test compound was significantly inhibited the viral neuraminidase (NA) expression. ConclusionThe present study well documented that the test compound was found to be a potent inhibitor of pandemic influenza A/H1N1 (2009) virus replication in MDCK cells. Further viral inhibitory studies were evident that the 4-methyl pyrimido (5, 4-c) quinoline-2,5(1H, 6H)-dione could be effective against influenza viral replication.