Abstract
Ethanol at 0.33% completely inhibited growth of Chlorella vulgaris after about 30 hr, causing increases in cellular concentrations of triglycerides, free sterols, and phosphatidylcholine. The ratio of linoleate to linolenate decreased in the triglyceride and free fatty acid fractions, while the double bond index increased as a result of ethanol treatment. Combined treatments of ethanol and [4-chloro-5-(dimethylamino)-2-phenyl-3(2 H) pyridazinone (BASF 13-338) resulted in partial alleviation of growth inhibition and a reversal of the ethanol-induced lipid effects. In addition, BASF 13-338 caused a qualitative change in the sterol composition of the alga, resulting in the accumulation of sterol precursors. In as much as BASF 13-338 reportedly inhibits the synthesis of linolenate from linoleate and now affects sterol synthesis in C. vulgaris, it is possible that the alleviation of ethanol-induced stress could be via alteration of unsaturated fatty acid and/or sterol synthesis.
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