Abstract
Single-Molecule Localization Microscopy (SMLM) offers a ∼10-fold improvement in resolution over conventional, diffraction-limited fluorescence microscopy, at the expense of acquisition time, data volume, and analysis overhead. For (d)STORM/(F)PALM, raw series typically require 10,000 to 100,000 frames acquired at 50 frames per second (FPS), meaning a day of diligent manual imaging yields only on the order of ten fields of view. Efforts have been made to ease the tedium of acquisition by automating SMLM microscopes.
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