Putative molecular mechanism underlying sperm chromatin remodelling is regulated by reproductive hormones

Manjeet Kaur Gill-Sharma,Jyoti Choudhuri,Serena D’Souza,Mukhtar Aleem Ansari

doi:10.1186/1868-7083-4-23

Manjeet Kaur Gill-Sharma, Jyoti Choudhuri + Show 2 more

Open Access

https://doi.org/10.1186/1868-7083-4-23

Copy DOI

Abstract

BackgroundThe putative regulatory role of the male reproductive hormones in the molecular mechanism underlying chromatin condensation remains poorly understood. In the past decade, we developed two adult male rat models wherein functional deficits of testosterone or FSH, produced after treatments with 20 mg/Kg/d of cyproterone acetate (CPA) per os, for a period of 15 days or 3 mg/Kg/d of fluphenazine decanoate (FD) subcutaneously, for a period of 60 days, respectively, affected the rate of sperm chromatin decondensation in vitro. These rat models have been used in the current study in order to delineate the putative roles of testosterone and FSH in the molecular mechanism underlying remodelling of sperm chromatin.ResultsWe report that deficits of both testosterone and FSH affected the turnover of polyubiquitylated histones and led to their accumulation in the testis. Functional deficits of testosterone reduced expression of MIWI, the 5-methyl cap binding RNA-binding protein (PIWIlike murine homologue of the Drosophila protein PIWI/P-element induced wimpy testis) containing a PAZ/Piwi-Argonaut-Zwille domain and levels of histone deacetylase1 (HDAC1), ubiquitin ligating enzyme (URE-B1/E3), 20S proteasome α1 concomitant with reduced expression of ubiquitin activating enzyme (ube1), conjugating enzyme (ube2d2), chromodomain Y like protein (cdyl), bromodomain testis specific protein (brdt), hdac6 (histone deacetylase6), androgen-dependent homeobox placentae embryonic protein (pem/RhoX5), histones h2b and th3 (testis-specific h3). Functional deficits of FSH reduced the expression of cdyl and brdt genes in the testis, affected turnover of ubiquitylated histones, stalled the physiological DNA repair mechanism and culminated in spermiation of DNA damaged sperm.ConclusionsWe aver that deficits of both testosterone and FSH differentially affected the process of sperm chromatin remodelling through subtle changes in the ‘chromatin condensation transcriptome and proteome’, thereby stalling the replacement of ‘dynamic’ histones with ‘inert’ protamines, and altering the epigenetic state of condensed sperm chromatin. The inappropriately condensed chromatin affected the sperm chromatin cytoarchitecture, evident from subtle ultrastructural changes in the nuclei of immature caput epididymal sperm of CPA- or FD-treated rats, incubated in vitro with dithiothreitol.

Highlights

The putative regulatory role of the male reproductive hormones in the molecular mechanism underlying chromatin condensation remains poorly understood
Reproductive hormone deficits adversely affect histone levels in maturing spermatids and epididymal sperm nuclear ultrastructure Basic proteins extracted from testis of control, fluphenazine decanoate (FD) and cyproterone acetate (CPA)-treated rats were resolved on continuous 15% acid urea polyacrylamide gels
It is evident from the presence of sperm with chromatin condensation defects in the caput epididymides of CPA- and FD-treated rats, that functional deficits of testosterone or follicle stimulating hormone (FSH) have adversely affected the process of chromatin condensation in the maturating elongating spermatids in at least some of the seminiferous tubules

Summary

Introduction

The putative regulatory role of the male reproductive hormones in the molecular mechanism underlying chromatin condensation remains poorly understood. We developed two adult male rat models wherein functional deficits of testosterone or FSH, produced after treatments with 20 mg/Kg/d of cyproterone acetate (CPA) per os, for a period of 15 days or 3 mg/Kg/d of fluphenazine decanoate (FD) subcutaneously, for a period of 60 days, respectively, affected the rate of sperm chromatin decondensation in vitro. These rat models have been used in the current study in order to delineate the putative roles of testosterone and FSH in the molecular mechanism underlying remodelling of sperm chromatin. That H3 di-acetylation at positions 9/14 is necessary for the condensation of chromatin, became evident in Pygopus mutant mice, which are infertile [26]

Methods

Results

Discussion

Conclusion