Abstract
Putative genes crtE, crtB, and crtI from Deinococcus wulumiqiensis R12, a novel species, were identified by genome mining and were co-expressed using the optimized Shine-Dalgarno (SD) regions to improve lycopene yield. A lycopene biosynthesis pathway was constructed by co-expressing these three genes in Escherichia coli. After optimizing the upstream SD regions and the culture medium, the recombinant strain EDW11 produced 88mg lycopene g(-1) dry cell wt (780mg lycopene l(-1)) after 40h fermentation without IPTG induction, while the strain EDW without optimized SD regions only produced 49mg lycopene g(-1) dry cell wt (417mg lycopene l(-1)). Based on the optimization of the upstream SD regions and culture medium, the yield of the strain EDW11 reached a high level during microbial lycopene production until now.
Published Version
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