Abstract
Reversed-phase liquid chromatography (RPLC) may have historical popularity, but it is not the only LC technique available to chromatographers. To help balance the choice of chromatographic technique, a discussion of the merits of being flexible when making the choice of LC technique for a given application is presented. Because having a flexible perspective is particularly important for biotherapeutics, it is important that choosing which technique to use is determined based on good science and begins with a physicochemical property assessment of the analytes, followed by subsequent alignment with the most appropriate retentive mode. An Earth-based figurative analogy is used to help highlight several popular LC techniques.
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