Abstract
The effect of puromycin on the intestinal absorption of cholesterol has been studied in rats with indwelling catheters in the left thoracic lymphatic duct. Puromycin administration to female rats produced a marked depression of cholesterol absorption under conditions where the absorption of simultaneously administered fatty acid was also dramatically inhibited. The same treatment of male rats also produced a significant depression in cholesterol absorption, but was without effect on absorption of the fatty acid. Despite the depressions of lipid absorption in puromycin-treated animals, there was no accumlation of either cholesterol or fatty acid in the intestinal mucosa of either sex. Actinomycin D treatment of fasting male and female rats, receiving constant infusions of saline, had no effect on the rate of lymph production. This suggest that altered lymph production was not responsible for the depressed lipid absorption observed in fed animals treated with protein synthesis inhibitors. The selective inhibition of cholesterol absorption in male rats also precludes the possibility that the major effect of the inhibitor is on delayed gastric emptying.
Highlights
The effect of puromycin on the intestinal absorption of cholesterol has been studied in rats with indwelling catheters in the left thoracic lymphatic duct
The present report represents our initial study on the effects of the protein synthesis antagonist, puromycin, on the intestinal absorption of exogenous cholesterol
It is one of the few studies comparing the effects of puromycin on lipid absorption in male and female rats, it is generally recognized that, with respect to lipid transport systems, female animals are more responsive to protein antagonists than are males
Summary
Puromycin and actinomycin D were obtained from Nutritional Biochemical Corporation, Cleveland, OH; the lipids were from Supelco, Bellefonte, PA; and the radioactive materials were purchased from Amersham-Searle Corporation, Arlington Heights, IL. Cholesterol and [7a-3H]cholesterol were purified through the dibromide and checked by thin-layer silicic acid chromatography
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