Purinergic signaling in mesenteric endothelial cells and peritoneal macrophages in chronic inflammation

Abstract

Event Abstract Back to Event Purinergic signaling in mesenteric endothelial cells and peritoneal macrophages in chronic inflammation Suellen D Arc Dos Santos Oliveira1, 2*, Nathalia F. Oliveira2, José Roberto Meyer Fernandes3, Claudia Lucia Martins Da Silva2 and Robson Coutinho Silva1 1 Federal University of Rio de Janeiro, Institute of Biophysics Carlos Chagas Filho, Brazil 2 Federal University of Rio de Janeiro, Institute of Biomedical Sciences, Brazil 3 Federal University of Rio de Janeiro, Institute of Biochemistry, Brazil Introduction Schistosomiasis causes a chronic inflammation related with physiological vascular alterations. Our group evaluated the immunomodulation of the purinergic signaling in mesenteric endothelial cells (MECs) and macrophages from S. mansoni-infected mice. Methods: The P2X7 receptor (P2X7R) function was evaluated by ATP-inducing membrane permeabilization to ethidium bromide (BE) measured by flow cytometry in MECs and macrophages. P2X7R expression was accessed by Immunocytochemistry and Western blotting. P2X7R-mediated nitric oxide (NO) production and ectonucleotidase activity were evaluated in MECs. Results: Schistosomiasis reduced the P2X7R expression in MECs and macrophages. We observed that 3 mM ATP was less efficient in inducing BE-uptake in MECs from infected (25 ± 2%; n=8) than uninfected mice (43 ± 1%, n=10). The selective P2X7R agonist BzATP induced a smaller NO synthesis in MECs from infected than control mice (5 ± 2% and 22 ± 2%, n=15, respectively). On the other hand, the ectonucleotidase activity was higher in MECs from infected than control mice (17 ± 3 and 7 ± 1 pmol Pi/µg of protein, n=16-14, respectively). The infection reduced the BE-uptake induced by 1 mM ATP in macrophages (95 ± 2% and 54 ± 6%, infected and uninfected mice respectively, n=12). Preliminary results also showed a reduced BzATP-mediated Ca2+ mobilization and increase of anti-inflammatory cytokine TGF-β in macrophages from infected-mice. Conclusion: Altogether, our data indicate that schistosomiasis modulates P2X7-receptor signaling in two cells types important to immune response. This could limit vascular inflammation and morbidity. Financial support: CNPq, FAPERJ-PRONEX, FAPERJ, INCT-INPeTAm/CNPq/MCT Keywords: Endothelial Cells, Macrophages, Schistosoma mansoni, purinergic signaling, chronic inflammation Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Host-pathogen interactions Citation: Dos Santos Oliveira S, Oliveira NF, Meyer Fernandes J, Martins Da Silva C and Coutinho Silva R (2013). Purinergic signaling in mesenteric endothelial cells and peritoneal macrophages in chronic inflammation. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00589 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 31 May 2013; Published Online: 22 Aug 2013. * Correspondence: Ms. Suellen D Arc Dos Santos Oliveira, Federal University of Rio de Janeiro, Institute of Biophysics Carlos Chagas Filho, Rio de Janeiro, Rio de Janeiro, Brazil, darcsu@hotmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Suellen D Arc Dos Santos Oliveira Nathalia F Oliveira José Roberto Meyer Fernandes Claudia Lucia Martins Da Silva Robson Coutinho Silva Google Suellen D Arc Dos Santos Oliveira Nathalia F Oliveira José Roberto Meyer Fernandes Claudia Lucia Martins Da Silva Robson Coutinho Silva Google Scholar Suellen D Arc Dos Santos Oliveira Nathalia F Oliveira José Roberto Meyer Fernandes Claudia Lucia Martins Da Silva Robson Coutinho Silva PubMed Suellen D Arc Dos Santos Oliveira Nathalia F Oliveira José Roberto Meyer Fernandes Claudia Lucia Martins Da Silva Robson Coutinho Silva Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.