Leukocyte adhesion to mesenteric endothelial cells mediated by P2Y1 receptor activation during schistosomiasis

Abstract

Event Abstract Back to Event Leukocyte adhesion to mesenteric endothelial cells mediated by P2Y1 receptor activation during schistosomiasis Suellen DArc Dos Santos Oliveira1, 2*, Nathalia Ferreira Oliveira2, Robson Coutinho-Silva1 and Claudia Lucia Martins Da Silva2 1 Federal University of Rio de Janeiro, Instituto de Biofísica Carlos Chagas Filho, Brazil 2 Federal University of Rio de Janeiro, Instituto de Ciências Biomédicas, Brazil Introduction: Previous work showed a downregulation of endothelial purinergic P2X7 receptor during chronic inflammation associated with schistosomiasis (Oliveira et al., 2013). We also observed an increased ectonucleotidase enzymatic activity, which produces ADP, an agonist of P2Y1 receptor (P2Y1R) that stimulates leukocyte adhesion to endothelial cells. Present work evaluated P2Y1R signaling in mesenteric endothelial cells (MECs) during schistosomiasis. Methods: Cultured MECs and mononuclear leukocytes (control and infected mice) were obtained as previously described (Oliveira et al., 2011). Western blotting assays investigated MECs P2Y1R expression and fluorimetric assays evaluated intracellular Ca2+. We used the agonist 2-MeSATP in the absence or presence of the selective antagonist MRS2179 (0.3 µM). Results: In the control group, 2-MeSATP (30 and 60 µM) increased leukocyte adhesion from 9.4 ± 1.3 to 24.5 ± 1.6 and 28.7 ± 1.7 cells/field, respectively (n=26–36 replicates, P<0.01) and MRS2179 prevented cell adhesion (9.2 ± 1.2 cells/field, n=36). However, in the inflammation model, there was an increased spontaneous adhesion (22 ± 2.0 cells/field, n=33), and the agonist had no significant effect. No alteration of P2Y1R expression was observed, but, the antagonist MRS2179 reduced basal adhesion (9.6 ± 1.0 cells/field, n=33), i.e., in the absence of 2-MeSATP. One possible explanation is that P2Y1R could have been previously activated by locally produced ADP. Conclusion: Schistosomiasis primes MECs in vivo which keep the acquired phenotype in vitro. These data indicate that schistosomiasis activates MECs P2Y1R signaling and consequently, leukocyte adhesion. References Oliveira, S.D.S. ; Quintas, Luis E.M. ; Amaral, Luciana S. ; Noël, François ; Farsky, Sandra H. ; Silva, Claudia L. M.. Increased Endothelial Cell-Leukocyte Interaction in Murine Schistosomiasis: Possible Priming of Endothelial Cells by the Disease. Plos One, v. 6, p. e23547, 2011. Oliveira, S.D.S. ; COUTINHO-SILVA, R. ; SILVA, C.L.M. . Endothelial P2X7 receptors expression is reduced by schistosomiasis. Purinergic Signalling (Print), v. 9, p. 81-89, 2013. Keywords: Endothelial Cells, Leukocytes, purinergic signaling, Schistosomiasis, chronic inflammation Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Host-pathogen interactions Citation: Oliveira S, Oliveira N, Coutinho-Silva R and Silva C (2013). Leukocyte adhesion to mesenteric endothelial cells mediated by P2Y1 receptor activation during schistosomiasis. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00463 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 17 Apr 2013; Published Online: 22 Aug 2013. * Correspondence: Mrs. Suellen DArc Dos Santos Oliveira, Federal University of Rio de Janeiro, Instituto de Biofísica Carlos Chagas Filho, Rio de Janeiro, Rio de Janeiro, Brazil, darcsu@hotmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Suellen DArc Dos Santos Oliveira Nathalia Ferreira Oliveira Robson Coutinho-Silva Claudia Lucia Martins Da Silva Google Suellen DArc Dos Santos Oliveira Nathalia Ferreira Oliveira Robson Coutinho-Silva Claudia Lucia Martins Da Silva Google Scholar Suellen DArc Dos Santos Oliveira Nathalia Ferreira Oliveira Robson Coutinho-Silva Claudia Lucia Martins Da Silva PubMed Suellen DArc Dos Santos Oliveira Nathalia Ferreira Oliveira Robson Coutinho-Silva Claudia Lucia Martins Da Silva Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.