Abstract
The relative contribution of denovo and salvage pathways for nucleotide synthesis in neoplastic tissues is one of the important factors in cancer chemotherapy. Although almost all antipurine and antipyrimidine I metabolites aim at denovo pathways as target sites, our previous investigations have suggested the possible role of salvage pathways in circumventing the action of the inhibitors of denovo synthesis (1-4). For purine nucleotide synthesis the denovo and the salvage synthetic enzymes, amidophosphoribosyltransferease (EC 2.4.2.14), adenine phosphoribosyl-transferase (APRT; EC 2.4.2.7) and hypoxanthine-guanine phosphoribosyl-transferase (HGPRT; EC 2.4.2.8), share the common substrate, PRPP, and are inhibited by the end products, purine nucleotides (5-7). These facts suggest that the inhibition of purine denovo synthesis might be compensated by purine salvage synthesis in chemotherapy. The purpose of this study was to compare the potential synthetic capactities of purine denovo and salvage pathways in neoplastic cells as reflected in enzyme speific activities and incorporation rates of [14C]formate, [14C]adenine, [14C] hypoxanthine and [l4C]guanine into purine nucleotides and nucleic acids.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.