Abstract
Ustiloxins are cyclopeptide mycotoxins produced by Villosiclava virens, the pathogenic fungus of rice false smut disease. Both resins SP207 and SP700 were screened to show the best adsorption and desorption properties for ustiloxins A and B among 20 commercial macroporous resins. Dynamic adsorption and desorption tests were carried out to optimize the process parameters. The optimal conditions for adsorption of resin SP207 were a processing volume as 32 bed volumes (BV), pH value of 4, and flow rate of 2 BV/h; and those for desorption of resin SP207 were a 40:60 (v/v) ratio of ethanol to water, an eluent volume of 4 BV, pH value of 4 and a flow rate of 3 BV/h. The optimal conditions for adsorption of resin SP700 were a processing volume of 26 BV, pH value as 4, flow rate of 2 BV/h; and those for desorption of resin SP700 were a 30:70 (v/v) ratio of ethanol to water solution as eluent, volume of 4 BV, pH value as 4 and flow rate of 2 BV/h. Under the optimal conditions; the purities of ustiloxins A and B obtained with resin SP207 increased 23.06-fold and 19.78-fold, respectively; and their recoveries were 96.67% and 81.25%; respectively. Similarly; the purities of ustiloxins A and B obtained with resin SP700 increased 14.75-fold and 15.33-fold and their recoveries were 93.65% and 88.64%; respectively. The results show that adsorption and desorption on SP207 and SP700 resins are effective strategies for purifying ustiloxins A and B. The developed methods are beneficial for large-scale preparation and purification of ustiloxins A and B from rice false smut balls.
Highlights
Ustiloxins are 13-membered cyclopeptides isolated from the water extract of the rice false smut disease pathogen Villosiclava virens Tanaka & Tanaka [1,2,3].They are toxic to plants and animals, especially due to their antimitotic activity, by inhibiting microtubule assembly and cell skeleton formation [4]
The results showed that the best desorption performance was obtained at a flow rate of
Absorption ratio: Desorption ratio: where Qe is the adsorption capacity at adsorption equilibrium; A and D are the adsorption ratio (%) and desorption ratio (%), respectively; C0 and Ce are the initial and equilibrium concentrations of ustiloxins in the solutions, respectively; Cd is the concentration of the ustiloxins in the effluent solutions of desorption; Vi is the volume of the initial sample solution; Vd is the volume of the desorption solution; W is the weight of the dry resin (g)
Summary
Ustiloxins are 13-membered cyclopeptides isolated from the water extract of the rice false smut disease pathogen Villosiclava virens Tanaka & Tanaka (anamorph: Ustilaginoidea virens Takahashi) [1,2,3]. They are toxic to plants and animals, especially due to their antimitotic activity, by inhibiting microtubule assembly and cell skeleton formation [4]. To the best of our knowledge, there was no information available about simultaneous enrichment and purification of ustiloxins A and B from rice false smut balls by using macroporous resins. The process parameters of two selected resins SP207 and SP700 were further optimized to purify ustiloxins A and B from rice false smut balls
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