Abstract
Optimization of four factors, namely methanol concentration ( A ), extraction pH value ( B ), material-to-solvent ratio ( C ) and extraction times ( D ), for extraction of ustiloxins A and B from rice false smut balls was achieved by using an L 18 orthogonal array design with three levels and four factors. The results show that the optimum conditions for ustiloxins A and B extraction should be A 1 B 2 C 2 D 3 corresponding to methanol concentration at 10%, extraction pH value at 6, material-to-solvent ratio at 1:30 (g/mL) and extraction times as 3, respectively. Under the optimum extraction condition, the content of ustiloxins A and B in rice false smut balls was analyzed to be 0.80 and 0.57 mg/g, respectively, on a dry weight basis. Keywords: Rice false smut balls, Villosiclava virens , Ustilaginoidea virens , ustiloxins A and B, orthogonal array design, extraction optimization. African Journal of Biotechnology , Vol 13(38) 3912-3918
Highlights
A and B from rice fa alse sm mut ba alls usiing an hogona al array y design orth
Optimizatio on of four fa actors, name ely methanoll concentrat ion (A), extrraction pH v value (B), ma aterial-tosolvent ratiio (C) and ex xtraction times (D), for ex xtraction of ustiloxins A and B from rice false smut balls was achiev ved by using g an L18 orth hogonal arra ay design with three leve els and fourr factors
Th he results show that the t optimum m conditions for ustiloxin ns A and B e extraction sh hould be A1B2C2D3 corres sponding to methano ol concentrattion at 10%, extraction pH
Summary
A and B from rice fa alse sm mut ba alls usiing an hogona al array y design orth. Optimizatio on of four fa actors, name ely methanoll concentrat ion (A), extrraction pH v value (B), ma aterial-tosolvent ratiio (C) and ex xtraction times (D), for ex xtraction of ustiloxins A and B from rice false smut balls was achiev ved by using g an L18 orth hogonal arra ay design with three leve els and fourr factors. Four factors, namely methanol concentration (that is, 10, 30 and 70%, v/v), extraction pH value (that is, 2, 6 and 10), material-to-solvent ratio (that is 1:20, 1:30 and 1:40, g/mL), and extraction times (that is, 1, 2 and 3)
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