Abstract
Methods were developed for the chromatography of fatty acid methyl esters on urea-impregnated papers, thin layers and columns of urea and Celite. Fatty acids of cholesterol esters from beef liver were separated by clathrate chromatography into factions of unsaturated, branched chain saturated and straight chain saturated fatty acids. Cholesterol esters were synthesized from these fractions and commercial cholesterol and their effect was tested on the incorporation of labelled amino acids into s-RNA of rat liver in vitro. Only esters of the fraction of branched chain fatty acids were active. This fraction was further separated into 6 components by preparative GLC. The cholesterol ester of only one of these components, a saturated fatty acid with carbon number 16.7, showed a stimulating effect on protein synthesis. This fatty acid was purified to 98–99% by repeated preparative GLC and obtained in crystalline form. It is concluded that the combination of clathrate chromatography and preparative GLC is very suitable for the isolation of pure branched chain fatty acids from biological materials.
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