Abstract
A precise study of the nature of viruses is rendered difficult by various extraneous substances. Although these substances do not appear to affect the action of the virus on its specific host, they modify the behavior of the virus in tests designed to ascertain its nature. Recently two methods have been reported for purifying viruses. One is based on ultrafiltration,1 the other on adsorption and elution.2The latter procedure is more easily carried out in the laboratory. However, it is difficult to standardize the technique and hence some authors have reported positive, others negative results. This paper deals with the standardization of the technique and the further purification of B. coli bacteriophage.Media and Reaction: The character of the medium influences the adsorption, elution and amount of phage produced. In a synthetic medium the concentration of phage at the outset is low and elution is unsatisfactory. Adsorption is best in an acid and elution in an alkaline medium. The following protocol is t...
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