Abstract
VIRAL material used to prepare inactivated virus vaccines must be concentrated to provide sufficient antigenic mass to stimulate the vaccine's protective mechanisms, and purified to remove nonviral host material which may cause side reactions and sensitization to host (for example, egg) components. A continuous-flow zonal centrifuge, the K-II-B1,2, has been designed for use in large scale production of influenza vaccines. We have grown influenza virus in tissue culture and have concentrated and purified it in a single step process using this centrifuge.
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