Abstract
Two of the main gangliosides in human milk were purified by silica gel (230–400 mesh) column chromatography. The gangliosides were identified as G D3 and G M3 by methanolysis (2 M hydrochloric acid; 60 or 85°C) and gas chromatography of trifluoroacetate derivatives on a fused-silica capillary column. The molar ratios of galactose, glucose and sialic acid were 1:1:2 and 1:1:1, respectively, and the sequence in both gangliosides comprised sialic acid—galactose—glucose—ceramide, as indicated by the time course of cleavage of individual components during methanolysis at 60° C.
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