Abstract
This chapter discusses purification of human and rat cytochromes P-450 and P-450 reductase and their reconstitution into phospholipid vesicles. In light of the enormous use of rats in the testing of drugs for toxicity and carcinogenicity, it is especially important to investigate differences between humans and rats in their metabolic routes and in the molecular structure of their cytochromes P-450 and cytochrome P-450 reductases. Several different forms of human and rat cytochromes P-450 have been purified and reconstituted together with the corresponding cytochrome P-450 reductases into phospholipids vesicles. The proteins in these vesicles have a membrane environment similar in structure and function to that in the endoplasmic reticulum. This chapter describes a purification technique for human cytochromes P-450 and cytochrome P-450 reductase, the technique of reconstituting these proteins into phospholipid vesicles.
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