Abstract
A two-step method for rat haptoglobin purification has been devised, which operates throughout under mild conditions (4°C, K +- or Na +-phosphate buffer, without salting out or precipitation at the isoelectric point, respectively). The two steps consist of adsorption chromatography on DEAE-Sephadex A-50 (first) and gel filtration on Sephadex G-150 (second). This method provides both high yield (about 90%) and high purity (practically, 100% rat haptoglobin). Moreover, it takes a short time of 4 days, which includes the time to recycle the impure material in the Sephadex G-150 column.
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