Abstract
The photosynthetic bacterium R. sphaeroides expresses two flagellar systems that are encoded by two complete gene clusters that have distinct phylogenetic origins. The isolation and purification of the Filament-Hook Basal Body (F-HBB) or the Hook Basal Body (HBB) structure is a troublesome task given the complexity of this nano-machine that is composed of multiple loosely bound substructures that can be lost during the isolation and purification procedure. A successful procedure requires adjustments to the standard method established for Salmonella. In this chapter, we describe a detailed protocol to isolate and purify the Fla2 F-HBB and HBB from R. sphaeroides a photosynthetic bacterium that has a complex intracellular membrane system that frequently interferes with isolation of high-quality samples.
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