Abstract
The cytoplasmic leucyl-tRNA synthetase from Euglena gracilis has been purified to homogeneity. The purification procedure includes chromatography on hydroxyapatite and on phosphocellulose, followed by Sepharose-Blue Dextran affinity chromatography and allows a 1030-fold purification of the enzyme with a recovery of 19% of the initial activity. The purified leucyl-tRNA synthetase (LeuRS) is a monomer of M r 116 000. The affinity constants of the enzyme are 2.4 · 10 −5 M for L-leucine, 1.1 · 10 −5 M for ATP, 1.6 · 10 −6 for Euglena tRNA Leu and 2 · 10 −7 M for yeast tRNA 3 Leu.
Full Text 
Sign-in/Register to access full text options
Sign-in/Register to access full text options 
Published version (
Free)
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
