Year-end Sale % OFF 
Abstract
Alpha Fetoprotein (AFP) is a major serum protein in the developing fetus and is of clinical significance as it is an oncofetal protein being synthesized by fetal organs and malignant tumors. AFP is here used as a diagnostic marker for hepatic carcinomas. In view of structural homology and similarities in physico-chemical properties with serum albumin, the separation and purification of AFP has always been a problem. Immunologically active AFP has been purified from human cord plasma using pseudoaffinity chromatography based on Cibacron blue substituted Sephadex G-100. AFP was quantified using rocket immunoelectrophoresis and double sandwich ELISA. Polyclonal antibodies were raised against purified AFP in mice. The purified antibodies were conjugated with peroxidase for use in double antibody sandwich ELISA. Purification of AFP from human cord plasma by an improved method with 55% recovery is reported.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
