Abstract
Abstract The sigma subunit of DNA-dependent RNA polymerase from vegetative Bacillus subtilis has been purified to at least 90% purity. Purified sigma binds to core RNA polymerase and stimulates in vitro transcription of phage Oe DNA by core RNA polymerase in the presence of up to 0.2 m KCl. Sigma also stimulates RNA synthesis to different extents with phage T4 DNA and poly(dA-dT) as templates, but this stimulation is markedly sensitive to increasing ionic strength. At 0.2 m KCl sigma subunit fails to stimulate transcription of T4 DNA and actually inhibits the synthesis of RNA with poly(dA-dT) as template.
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