Abstract
Rhizome of Ligusticum chuanxiong is an effective medical plant, which has been extensively applied for centuries in migraine and cardiovascular diseases treatment in China. Polysaccharides from this plant have been shown to have interesting bioactivities, but previous studies have only been performed on the neutral polysaccharides. In this study, LCP-I-I, a pectic polysaccharide fraction, was obtained from the 100 °C water extracts of L. chuangxiong rhizomes and purified by diethylaminethyl (DEAE) sepharose anion exchange chromatography and gel filtration. Monosaccharide analysis and linkage determination in addition to Fourier transform infrared (FT-IR) spectrometer and Nuclear magnetic resonance (NMR) spectrum, indicated that LCP-I-I is a typical pectic polysaccharide, with homo-galacturonan and rhamnogalacturonan type I regions and arabinogalactan type I and type II (AG-I/AG-II) side chains. LCP-I-I exhibited potent complement fixation activity, ICH50 of 26.3 ± 2.2 µg/mL, and thus has potential as a natural immunomodulator.
Highlights
Chuanxiong Rhizoma (Chuanxiong, CX), the dried rhizome of Ligusticum chuanxiong Hort (Umbelliferae), is an effective medical plant, which has been extensively applied for centuries in migraine and cardiovascular diseases treatment in China [1]
L. chuanxiong polysaccharides focus on the extraction techniques
The monosaccharide composition present in LCP-I-I is typical for a pectic polysaccharide, consisting of galactose (Gal), composition present in LCP-I-I is typical for a pectic polysaccharide, consisting of galactose (Gal), galacturonic acid (GalA), arabinose (Ara) and rhamnose (Rha)
Summary
Chuanxiong Rhizoma (Chuanxiong, CX), the dried rhizome of Ligusticum chuanxiong Hort (Umbelliferae), is an effective medical plant, which has been extensively applied for centuries in migraine and cardiovascular diseases treatment in China [1]. Polysaccharides isolated from L. chuanxiong have been shown to have several bioactivities, such as Polysaccharides isolated from L. chuanxiong have been shown to have several bioactivities, such antioxidant activity [2,9], anticancer [10] and antibacterial activity [11,12]. L. chuanxiong polysaccharides focus on the extraction techniques These techniques include the following following methods: microwave [13], pectinase treatment [14], cellulose enzyme treatment [15], methods: microwave [13], pectinase treatment [14], cellulose enzyme treatment [15], ultrasonic-assisted ultrasonic-assisted extraction [11,16] and boiling water extraction [2]. They are have been characterized, and they are all neutral polysaccharides They are only related to only related to monosaccharide compositions and molecular weight [2,17,18].
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