Abstract
From the complex enzyme preparation Bruzaime BGX, β-glucanase enzyme with a specific activity of 369.5 units/mg of protein and a degree of purification of 57.7 was isolated by ethanol precipitation and purified by gel filtration on Sephadex G-25 and G-150 and ion-exchange chromatography on DEAE - cellulose. The influence of temperature and pH on the activity and stability of the enzyme was studied in the temperature range 30–70°C and pH 4.0–7.0. It was found that the optimum effect of β-glucanase corresponds to pH 4.8 and a temperature of 56°C, the enzyme has a sufficiently high thermal and acid stability, hydrolyzes non-starch polysaccharides containing both β- 1,3 and β- 1,4 - glucosidic bonds, which makes its use in biotechnology promising for the hydrolysis of non-starch polysaccharides of grain.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: IOP Conference Series: Earth and Environmental Science
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
