Abstract
From the complex enzyme preparation Bruzaime BGX, β-glucanase enzyme with a specific activity of 369.5 units/mg of protein and a degree of purification of 57.7 was isolated by ethanol precipitation and purified by gel filtration on Sephadex G-25 and G-150 and ion-exchange chromatography on DEAE - cellulose. The influence of temperature and pH on the activity and stability of the enzyme was studied in the temperature range 30–70°C and pH 4.0–7.0. It was found that the optimum effect of β-glucanase corresponds to pH 4.8 and a temperature of 56°C, the enzyme has a sufficiently high thermal and acid stability, hydrolyzes non-starch polysaccharides containing both β- 1,3 and β- 1,4 - glucosidic bonds, which makes its use in biotechnology promising for the hydrolysis of non-starch polysaccharides of grain.
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