Abstract
Significant amounts of vitamin B12 (about 116.3∼556.3 µg/100 g) were determined in a Japanese salted and fermented salmon kidney “Mefun.” A corrinoid compound was purified to homogeneity from Mefun and partially characterized. TLC and HPLC patterns of the purified corrinoid compound were identical to those of authentic vitamin B12, but not to inactive corrinoids. The vitamin B12 found in Mefun was not derived from concomitant vitamin B12‐synthesizing bacteria, but had accumulated in the salmon kidney. Gel filtration experiments demonstrated that most of the vitamin B12 found in Mefun was recovered in the free vitamin B12 fractions. These results indicate that Mefun would be an excellent vitamin B12 (free form) source for elderly persons with food‐bound vitamin B12 malabsorption.
Published Version
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