Abstract
The purification of Japanese quail IgG from serum was performed using caprilic acid and ammonium sulfate, and from egg yolk using PEG-6000 and ethanol. After confirming the purification and the concentration of IgG, the yolk samples had twice the amount of protein compared to serum samples. The IgG extracts were analyzed by SDS-PAGE and western blot showing similar results as the ones of chicken IgG used as the standard. So, these methodologies can be used for purifying quail serum or egg yolk IgG, which would enable the development of diagnostic assays.
Highlights
After confirming the purification and the concentration of IgG, the yolk samples had twice the amount of protein compared to serum samples
The IgG extracts were analyzed by SDS-PAGE and western blot showing similar results as the ones of chicken IgG used as the standard
These methodologies can be used for purifying quail serum or egg yolk IgG, which would enable the development of diagnostic assays
Summary
Purificação de IgG de codorna a partir de soro e gema de ovos Os objetivos deste trabalho foram a avaliação e a purificação de IgG, em amostras de soro e gema de ovos de codorna, para futura utilização em testes sorológicos. A purificação de IgG da gema de ovos de codorna foi realizado segundo Akita e Nakai (1992), Abbassi et al (1999) e Bizhanov e Vyshniauskis (2000).
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