Abstract
Refrigeration plays a vital role in prolonging the marketing supply period of ‘Nanguo’ pear; however, it induces a loss of esters, which seriously affects the aromatic quality of the fruit. This study characterized the key enzyme genes and transcription factors closely correlated with ester loss of cold-stored ‘Nanguo’ pears. However, their transcriptional regulation is largely unknown. We found that the expression of PuLOX3, PuNAC100-like, and PubHLH61 was notably repressed and was accompanied by aroma fading in cold-stored ‘Nanguo’ pears. The overexpression or silencing of PuNAC100-like or PubHLH61 led to the overexpression or suppression of PuLOX3. Yeast-hybridization, electrophoretic mobility shift assay, and transient transfection analyses showed that the nuclear-localized PuNAC100-like directly bound to the PuLOX3 promoter successfully, activating its expression. Yeast two-hybrid and bimolecular fluorescence complementation indicated that PuNAC100-like interacted with PubHLH61. In addition, the transcriptional regulation of PubHLH61 on the PuNAC100-like was also observed. Collectively, these findings revealed that the suppressed expression of PuNAC100-like and PubHLH61 down-regulated the transcription of PuLOX3 under low temperature stress and thus induced a decrease in aroma quality in cold-stored ‘Nanguo’ pears.
Published Version
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