Abstract
There is growing demand for gentle technologies to improve the lipid bioaccessibility (BA) of Chlorella vulgaris biomass while preserving cell integrity and therefore oxidative stability. Pulsed electric field treatment (PEF, 5 μs at 20 kV cm−1, 31.8 kJ kg−1sus) led to an enhancement in lipid BA from 4–7.8% (untreated) to 18.7–20.9%. To reach such a level of BA, incubation in buffer after the treatment (12 h at 25/37 °C, 48 h at 4 °C) was required. As hypothesized, PEF preserved cell integrity, as shown by particle size and scanning electron microscopy analyses, as well as oxidative stability of the biomass over 3 months at 40 °C. Proteome analysis identified four proteins that may be involved in cell wall lytic activity during incubation after PEF. Future work should focus on further understanding the mechanism behind incubation after PEF and studying the potential effect played by endogenous cell wall-degrading enzymes.
Highlights
Microalgae are unicellular organisms that represent a source of several valuable nutrients, such as polyunsaturated fatty acids (PUFAs), protein and minerals
Proteome analysis identified four proteins that may be involved in cell wall lytic activity during incubation after Pulsed electric field (PEF)
Future work should focus on further understanding the mechanism behind incubation after PEF and studying the potential effect played by endog enous cell wall-degrading enzymes
Summary
Microalgae are unicellular organisms that represent a source of several valuable nutrients, such as polyunsaturated fatty acids (PUFAs), protein and minerals. Our previous study showed that the in vitro protein bioaccessibility of Chlorella was approximately 40–70%, whereas the lipid bioaccessibility was below 20% because it was limited by the indigestibility of the cell wall (Canelli, Murciano Martínez, Maude Hauser, et al, 2021; Canelli, Tarnutzer, et al, 2020). These results indicate a strong need for research aimed at improving the bio accessibility of lipids from C. vulgaris. To preserve biomass quality, research should focus on finding a disruption process that in creases the bioaccessibility of the lipid fraction while avoiding lipid oxidation
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Innovative Food Science & Emerging Technologies
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
