Pulse radiolysis study of rapid electron transfer from electron adduct and reducing OH adduct of dAMP to riboflavin and flavin adenine dinucleotide (FAD)

Abstract

AbstractRapid electron transfer from electron adduct and reducing OH adduct of 2‐deoxyadenosine‐5′‐monophosphate acid (dAMP) to riboflavin (RF) and flavin adenine dinucleotide (FAD) was studied using the time‐resolved pulse radiolysis techniques. Both spectroscope and kinetic analyses showed that transient absorption spectrum of electron adduct or OH adduct of dAMP formed at first, and men changed to that of radical anion of RF or FAD after several microseconds of pulse. The evidence indicated that electron transfer from electron adduct and reducing OH adduct of dAMP to RF or FAD did occur. From buildup or bleaching kinetics of radical anions of RF and FAD, the rate constants for electron transfer were determined, respectively.