Pulp interstitial fluid pressure and blood flow after denervation and electrical tooth stimulation in the ferret

Abstract

The effects of sensory and sympathetic denervation on simultaneously measured interstitial fluid pressure and blood flow in the canine pulp before, during and after electrical tooth stimulation were investigated in 25 ferrets. The micropuncture technique was used to measure interstitial fluid pressure and laser-Doppler flowmetry was used to record pulpal blood flow. Animals with an intact innervation (group 1) served as controls. Sensory denervation was by axotomy of the left inferior alveolar nerve 10 days before the experiment (group 2) and sympathectomy by removal of the left cervical ganglion 5 days before the experiments (group 3). The study was designed to verify whether denervation affected basal pulp blood flow and interstitial fluid pressures during control conditions and/or after tooth stimulation. During control conditions the interstitial fluid pressure averaged 1.32 ± 0.07 kPa in group l, whereas the mean was only 0.51 ± 0.13 kPa in the axotomized animals (group 2). The difference was highly significant, indicating decreased blood or interstitial fluid volume in the pulp after inferior alveolar nerve axotomy. In the sympathectomized group neither the interstitial fluid pressure nor the pulp blood flow was significantly different from those of group I. Electrical tooth stimulation caused an almost simultaneous increase in interstitial fluid pressure and pulp blood flow in groups I and 3, whereas stimulation did not significantly change either variable in the axotomized animals (group 2). It is concluded that a resting nervous vasodilator tone of sensory origin exists in the ferret dental pulp, and that the sensory nerves are responsible for the increased interstitial fluid pressure and pulp blood Row during tooth stimulation.