Abstract
A rapid method for Pullulan-stabilized silver nanoparticles (PuAgNPs) synthesis has been developed. Different concentrations of Pullulan and Silver nitrate and effects of reaction time, pH was used to investigate the synthesis of silver nanoparticles. The synthesized Pu-AgNPs were first screened and identified using surface plasmon peaks of UV–VIS spectroscopy.The research results indicated that the surface plasmon resonance peaks were observed between 410–460 nm wavelengths in UV-VIS spectroscopy studies.The morphology of the synthesized AgNPs proved a variation in spherical shape and polydispersed with an averagesize of 10-55 nm, using TEM. Further, five characteristic peaksconfirmed the presence of elemental silver and the crystalline structure of silver nanoparticles from XRD analysis. From FTIR spectra, stretching vibrations of hydroxyl (O-H), carbonyl (C=O) and C=C stretches exhibits the reduction and stabilization of AgNPs. Further, clear zones of inhibition (about 10-25 mm) against four bacterial pathogens obtained in the antibacterial studies for the synthesized PuAgNPs.The experimental results demonstrated that pullulan could be used as reducing and stabilizing agentfor formation of AgNPs and can be used as redoubtablebactericidal agents.
Highlights
Metal-based nanoparticles find tremendous applications in real time biomedical, nutrition and electronic industries
The synthesized Pu-AgNPs were characterized by UV-visible spectroscopy Transmission Electron Microscopy (TEM), X-Ray Diffraction (XRD) and Fourier Transform InfraRed (FTIR) spectroscopy
The synthesized Silver nanoparticles are in spherical shape with particle sizes between 10-55nm
Summary
Metal-based nanoparticles find tremendous applications in real time biomedical, nutrition and electronic industries. A new concept referred as ‘green’ synthesis of silver nanoparticles using polysaccharides excreted by fungi as renewable polymeric substances is used for stabilization of synthesized NPs (Raveendran et al, 2003). Many attempts were made by several researchers to study the effect of parameters like concentrations of Pullulan and silver nitrate, preparative (photo induced, electrochemical, solvothermal) methodsand temperature on the formation and growth of silver nanoparticles in the particle size of 2-40 nm. The influence of (increased) reaction time and change in pH was studied for direct reduction of silver nitrate with Pullulan to produce Pullulan-stabilized AgNPs. The synthesized Pu-AgNPs were characterized by UV-visible spectroscopy Transmission Electron Microscopy (TEM), X-Ray Diffraction (XRD) and Fourier Transform InfraRed (FTIR) spectroscopy. The antibacterial activity of Pu-AgNPs towards bactericidal agents was studied
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