Abstract
OBJECTIVETo evaluate the protective effect of puerarin [an isoflavone compound extracted from Gegen (Radix Puerariae Lobatae)] in scleral remodeling induced by extremely low frequency electromagnetic fields (ELF-EMFs). METHODSHuman fetal scleral fibroblasts (HFSFs) were divided into 5 groups: (1) untreated controls; (2) cells treated with ELF-EMFs; (3) cells treated with ELF-EMFs and puerarin 0.1 μM; (4) cells treated with ELF-EMFs and puerarin 1 μM; (5) cells treated with ELF-EMFs and puerarin 10 μM. Cell proliferation activity was measured by the cell-counting kit-8 assay. Matrix metalloproteinase-2 (MMP-2) activity was measured by gelatin enzymography. MMP-2 and collagenI(COL1A1) mRNA, protein expression were measured by Real-Time polymerase chain reaction, Western blot analysis, respectively. RESULTSPuerarin reduced the inhibition in cell proliferation, MMP-2 activity, mRNA, protein expression of HFSFs exposed to ELF-EMFs and enhanced the COL1A1 mRNA and protein expression. CONCLUSIONPuerarin was found to participate in the matrix remodeling process. It might be a potential agent for the treatment of extracellular matrix degradation of sclera associated with ocular conditions.
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