Abstract

Objective To observe the matrix metalloproteinase 2 (MMP-2) activity and protein expression in human fetal scleral fibroblasts (HFSFs) exposed to an extremely low frequency electromagnetic field (ELF-EMF) and its impact on collagen I synthesis. Methods This was an experimental study. HFSFs were cultured and divided into a control group, radiation group and inhibitor group according to their exposure to 50 Hz, 0.2 mT electromagnetic field or joint MMP-2 specific inhibitors (sc-204092) . MMP-2 activity was measured by gelatin enzymography. MMP-2 and collagen I protein expression were detected by Western Blot. A single factor analysis of variance was used to determine whether there was a difference among these groups. Results There were significant differences between the three groups (F=14.96, 139.88, 63.46, P<0.01) . Compared to the control group, MMP-2 activity and protein expression increased in the radiation group (P<0.05) and collagen I protein expression decreased (P<0.01) . Compared to the radiation group, MMP-2 activity and protein expression decreased in the inhibitor group (P< 0.01) and collagen I protein expression increased (P<0.05) . Conclusion ELF-EMF may regulate the synthesis of collagen I by affecting the activity and protein expression of MMP-2. Key words: Radiation, ionizing; Human fetal sclera fibroblast; Matrix metalloproteinase 2; Collagen type I

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