PSY153-MDR, a p12969-DIM-related mega plasmid carrying blaIMP-45 and armA, from clinical Pseudomonas putida.

Min Yuan,Dongsheng Zhou,Xiaofei Zhao,Juan Li,Jiao Feng,Jianguo Xu,Defu Zhang,Hai Chen,Jinxing Lu,Xiong Zhu,Xia Chen,Zhe Zhan

doi:10.18632/oncotarget.19496

Abstract

This work characterized mega plasmid pSY153-MDR, carrying blaIMP-45 and armA, from a multidrug-resistant (MDR) Pseudomonas putida isolate from the urine of a cerebral infarction patient in China. The backbone of pSY153-MDR was closely related to Pseudomonas plasmids p12969-DIM, pOZ176, pBM413, pTTS12, and pRBL16, and could not be assigned to any of the known incompatibility groups. The accessory modules of pSY153-MDR were composed of 10 individual insertion sequence elements and two different MDR regions, and differed dramatically from the above plasmids. Fifteen non-redundant resistance markers were identified to be involved in resistance to at least eight distinct classes of antibiotics. All of these resistance genes were associated with mobile elements, and were embedded within the two MDR regions. blaIMP-45 and armA coexisted in a Tn1403–Tn1548 region, which was generated from homologous recombination of Tn1403- and Tn1548-like transposons. The second copy of armA was a component of the ISCR28–armA–∆ISCR28 structure, representing a novel armA vehicle. This vehicle was located within In48, which was related to In363 and In1058. Data presented here provide a deeper insight into the evolutionary history of SY153, especially in regard to how it became extensively drug-resistant.

Highlights

Pseudomonas putida is a non-fermentative Gramnegative bacillus belonging to the fluorescent group of the genus Pseudomonas [1]
The current study presents the second fullysequenced MBL-encoding plasmid, designated pSY153-multidrug resistance (MDR), from clinical P. putida. pSY153-MDR was a 468.2-kb mega plasmid, and carried blaIMP-45 and armA, as well as additional markers involved in resistance to β-lactams, quinolones, macrolides, tetracyclines, amphenicols, quaternary ammonium compounds, sulphonamides, trimethoprim, and rifampicin
polymerase chain reaction (PCR) results demonstrated that strain SY153 harbored blaIMP, blaOXA, and armA, but none of the rest bla and 16S rRNA methylase genes screened for in this analysis, with the first two genes being confirmed as blaIMP-45 and blaOXA-1 by genomic sequencing

Summary

Introduction

Pseudomonas putida is a non-fermentative Gramnegative bacillus belonging to the fluorescent group of the genus Pseudomonas [1]. It is an opportunistic human pathogen, responsible for nosocomial infections in immunocompromised patients and in those with catheter or indwelling devices. Metallo-βlactamases (MBLs) are a group of β-lactamases that can hydrolyze all β-lactams, including carbapenems, except aztreonam [3]. Genes encoding these MBLs are generally associated with integrons and transposons, and often coexist with genes conferring resistance to other classes of antibiotics [4]. This association results from co-selection under the pressure of multiple antibiotics, and leads to multidrug resistance (MDR) in Pseudomonas strains [4]

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Results

Conclusion