PSIII-12 A Preliminary Evaluation on Novel Tryptophan Additive on Apparent Total Tract and Ileal Digesta of Nutrients in Growing Pigs Using Cannulation Technique

Abstract

Abstract Amino acids (AA) are the primary structure of proteins. Earlier studies proved that AA contents in the diet are closely related to efficiency of nutrient utilization in pigs. Therefore, we aimed to analyze the effect of tryptophan [(Trp), Trp-proline and calcium-Trp)] supplementation on the apparent total tract digestibility (ATTD) and apparent ileal digestibility (AID) of dry matter (DM), nitrogen (N), energy (GE), and amino acids (AAs) in eight growing pigs [(Landrace × Yorkshire) × Duroc)]. Pigs with average body weights of 25 kg were surgically inserted with post-valve T-caecum cannulation approximately 15 cm anterior to the ileo-cecal junction and randomly assigned to the following diets: CON (corn- soybean meal-distillers dried grain soluble -based basal diet), Trp Pro (CON + 20g/kg Trp Pro), Ca-Trp (CON+ 20g/kg Calcium Trp), and N-free diet using a 4× 4 Latin squares design in duplicates. The novel Trp supplements did not exhibit any significant difference in ATTD and AID of DM, GE, and N digestibility. However, the ATTD of essential AAs such as valine, isoleucine, phenylalanine, lysine, and tryptophan and some non-essential AA like serine, glutamic acid, and glycine was increased (P < 0.05) in novel Trp group. The inclusion of the novel Trp to the diet reduced (P > 0.05) ATTD of threonine but increased the AID of threonine. While other AAs showed the least response to the Trp supplementation. Also, ATTD of methionine tended to be greater in Trp group compared with the CON. In short, the inclusion of 20 g/kg modified form of novel Trp supplementations showed beneficial effects to enhance the AA deposits in the tissue of pigs without any adverse effect.