Abstract

Summary.The characteristics of 12 isolates from 7 different hosts which were believed to be Pseudomonas viridiflava were studied using a variety of cultural and biochemical tests; in nutritional tests, 135 compounds were screened for their ability to serve as sole carbon and energy (C/E) sources. All cultures were similar in character and resembled closely Burkholder's original isolates: they were distinguishable from 13 cultures of other phytopathogenic Pseudomonas spp. Although they closely resembled Ps. syringae, they could be distinguished by their ability to rot potato, to utilize d(—) tartrate as sole C/E source and by their failure to utilize sucrose and to form levan on 5% sucrose nutrient agar. Pseudomonas viridiflava is a common epiphyte at least on dwarf beans (Scarlett, 1968) though it has rarely been cited as a cause of disease in Phaseolus spp. Results of lesion tests in dwarf bean pods and in the tobacco hypersensitivity test together with pectolytic activity strongly suggest however that it is a potential pathogen.

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