PS-P16-4: VALIDATION OF CHO ET AL FORMULA FOR APOLIPOPROTEIN B100 CALCULATION; A POTENTIAL ATHEROSCLEROTIC RISK TOOL IN LIMITED RESOURCES SETTINGS

Abstract

Objectives: Increased atherogenic lipid molecules such as low-density lipoprotein cholesterol (LDL) concentration is associated with increased risk of atherosclerotic, but a substantial risk of cardiovascular disease often remains after LDL and other lipid parameters concentrations have been extensively treated to healthy level. Owning to the lack of assay procedure in the current research geographical area, this study aims to determine the sensitivity and specificity of Apolipoprotein B100 derived from Cho's formula in conjunction with existing routine lipid profile parameters. Method: The lipid profile parameters (TC, LDLC, HDLC, Apo B100, TG and non-HDL-C) in the fasting samples of 301 adult patients in the Chemical Pathology Laboratories of the Federal Medical Centre; Birnin Kudu Jigawa State, and Birnin Kebbi, Kebbi State Nigeria. The Apo B100 was calculated using Cho equation (Cho et al., 2012). Receiver Operating Curve (ROC) was employed to determine the sensitivity and reliability of lipid parameters in the assessment of atherosclerotic risk. Cho^’ s Equation: ApoB100 = -33.12 + 0.675*LDLc + 11.95*ln(Trig) Results: From the table 1, the areas under the curves represent the probability that the lipid assay for a randomly chosen atherosclerotic risk case (sensitivity) will exceed the result for a randomly chosen no risk case (specificity). The asymptotic significance for HDL-C, TC, LDL-C Apo B100 and non-HDL-C were less than 0.05, and AUC greater than 0.7. Furthermore, LDL-C, Apo B100, non-HDL-C, and TC have AUC of 1.00, 0.990, 0.976, and 0.885 respectively. In addition to their high AUC, the specificity of TC, HDL-C, LDL-C Apo B100 and non-HDL-C for ASCVD risk were 74.4, 37.2, 100.0, 94.8 and 88.4 percent respectfully, whereas TG has no significant influence (AUC) on risk of ASCVD. It is worthy to mention that the AUC of HDL-C was statistically significant, but lower than 0.70 (0.429). The serum TC, LDL-C, Apo B100, and non-HDL-C level of greater than 172.1mmg/dl, 106.3 mg/dl, 95.6 mg/dl, and 135.3 mg/dl respectively were associated with a high risk of atherosclerotic as indicated by their specificities and sensitivities. Conclusion: It can be concluded that using the TC, LDL-C, Apo B100 and non-HDL-C for atherosclerotic risk estimation guaranteed significant reliability. It can be concluded that Apo B100 derived using Cho formula showed a high sensitivity and specificity in predicting atherosclerotic risk at a serum level of approximately 96 mg/dl. Therefore, Apo B100 derived from Cho formula may be a useful tool in the management of atherosclerotic cardiovascular disease in limited resources settings.