PS 02-78 THE ASSOCIATION BETWEEN ESTRADIOL AND ESR1 GENE POLYMORPHISM IN POSTMENOPAUSAL WOMEN WITH ARTERIAL HYPERTENSION AND TYPE 2 DIABETES MELLITUS

Abstract

Objective: To determine associations between estradiol and ESR1 gene polymorphism in postmenopausal women with arterial hypertension and type 2 diabetes mellitus. Design and Method: The study included 137 women from Kharkiv region of Ukraine including patients with type 2 Diabetes Mellitus (T2DM) (1st group, n = 30), arterial hypertension (AH) of 2–3 degree (2nd group, n = 34), T2DM and AH of 2–3 degree (3rd group, n = 42) and 31 healthy volunteer (4th group). Serum levels of estradiol were evaluated by ELISA. Assays of estrogen receptor gene alpha (ESR1) were performed using restriction analysis of the amplification products of genome specific regions, i.e., promoter region T397C. Results: The levels of estradiol were the following in the studied patients: group 1 - (52.34 ± 1.15) pg/L, group 2 - (41.36 ± 1.16) pg/L, group 3 - (31.82 ± 1.69) pg/L and healthy volunteers - (55.30 ± 1.51)pg/L. Differences between group 1 and 2, 2 and 3, 1 and 3, patients with hypertension (groups 2 and 3 together) and healthy volunteers were significant (p < 0.05). Homozygote TT genotype of the ESR1 gene predominated in healthy donors 70.97 ± 8.15% (n = 22) compared to patients: group 1 - 10.00 ± 5.48% (n = 3), group 2 - 8.82 ± 4.86% (n = 3) and group 3 - 11.90 ± 5.00 (n = 5). Heterozygous TC genotype of the ESR1 gene was observed in 56.67 ± 9.05% (n = 17) of patients in group 1, 67.65 ± 8.02% (n = 23) in group 2, 61.90 ± 7.49% (n = 26) in group 3 and 12.90 ± 6.02% (n = 4) of healthy volunteers. Homozygous CC genotype of the ESR1 gene was found in 33.33 ± 8.61% (n = 10) of patients of group 1, 23.53 ± 7.27% (n = 8) in group 2, 26.19 ± 6.78% (n = 11) in group 3, and in 16.13 ± 6.61% (n = 5) of healthy volunteers. Differences between heterozygous TC genotype of the ESR1 gene in the 2nd and 3rd groups were significant (p < 0.05). Conclusions: Women with hypertension had significantly lower levels of serum estradiol than healthy female volunteers. This coincided with greater frequency of heterozygous TC polymorphism T397C of the ESR1 gene.