Abstract
PRR14 (Proline rich protein 14) was firstly identified for its ability to specify and localize heterochromatin during cell cycle progression. Aberrant expression of PRR14 is associated with the tumorigenesis and progression of lung cancer. However, its involvement in colon cancer remains unknown. Herein, we report the role of PRR14 in colon cancer. Colon cancer tissue microarray was used to analyze and compare the expression of PRR14 among some clinicopathological characteristics of colon cancer. HCT116 and RKO cells were transfected with siRNA to downregulate PRR14 expression. The roles of PRR14 in proliferation, migration and invasion of the cell lines were determined using cell counting kit-8, colony formation assay, wound healing assay and transwell assays respectively. The expression of PRR14 was measured using immunofluorescence, qRT- PCR and western blot. Epithelial-mesenchymal transition (EMT) markers were determined by western blot. PRR14 was highly expressed in colon cancer tissues, and the expression level was correlated with tumor size, distant metastasis and Tumor Node Metastasis stages. Functional study revealed that downregulation of PRR14 inhibited colon cancer cells growth, migration and invasion. Furthermore, knockdown of PRR14 inhibited epithelial-mesenchymal transition (EMT) process, cell cycle-associated proteins expression and p-AKT level. PRR14 may promote the progression and metastasis of colon cancer, and may be a novel prognostic and therapeutic marker for the disease.
Highlights
Colon cancer is one of the most aggressive digestive system cancers worldwide; and its incidence rate in China has been increasing dramatically over the years [1, 2]
PRR14 was highly expressed in colon cancer tissues, and the expression level was correlated with tumor size, distant metastasis and Tumor Node Metastasis stages
Functional study revealed that downregulation of PRR14 inhibited colon cancer cells growth, migration and invasion
Summary
Colon cancer is one of the most aggressive digestive system cancers worldwide; and its incidence rate in China has been increasing dramatically over the years [1, 2]. A large number of genes associated with the metastasis and recurrence of colon cancer have been identified, the molecular pathogenesis of this disease is still far from being fully elucidated. Identification of new genes associated with colon cancer progression is of great importance for the prevention, early and accurate diagnosis, and treatment of the disease. PRR14 (Proline Rich Protein 14) is a member of the proline-rich protein family. It contains one proline-rich region flanked by N- and C-terminal nuclear localization signals [3]. PRR14 (Proline rich protein 14) was firstly identified for its ability to specify and localize heterochromatin during cell cycle progression. Aberrant expression of PRR14 is associated with the tumorigenesis and progression of lung cancer. We report the role of PRR14 in colon cancer
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call