Abstract

Proton-electron double-resonance imaging (PEDRI) is an alternative method to EPR for detecting and imaging free radicals in biological samples or animals. PEDRI uses the Overhauser effect, and involves collecting a proton NMR image while irradiating the EPR of the free radical under study. The spatial resolution in PEDRI is not affected by the linewidth of the free radical’s EPR resonance. An important development of the technique is field-cycled PEDRI (FC-PEDRI), in which the applied magnetic field is switched between two levels during the pulse sequence in order to decrease non-resonant RF power deposition and to increase sensitivity. A spectroscopic version of FC-PEDRI, called field-cycled dynamic nuclear polarization (FC-DNP) is useful for studying low concentrations of free radicals and enables EPR spectra to be obtained via the Overhauser effect. This Chapter describes the techniques and practical implementation of PEDRI and related techniques. Applications of the methods are described, and likely future uses of the techniques are discussed.

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