Proteomic Profile Analysis of Tomato (Solanum lycopersicum L.) Roots Under Salinity Stress

Abstract

Salinity is a major constraint to crop productivity. This study aimed at a better understanding of the molecular adaptation mechanisms of salinity stress was carried out on tomato (Solanum lycopersicum L.) Early Urbana Vf cultivar, using proteomic analysis. Seedlings of tomato were cultivated in the hydroponic culture system. The salt stress (NaCl) was applied (0 mM and 90 mM) and sustained for two weeks. Protein content was extracted from the seedlings roots of both non-saline control and salt-stressed plants, and separated by two-dimensional gel electrophoresis. 22 protein spots were identified and classified into seven categories. Obtained results from two-dimensional gel electrophoresis and proteome analysis indicated that some proteins exhibited expression variations strictly related to salt stress which could be related to genotype tolerance or biochemical Compatibility. The proteins identified with up-regulation belonged to the Heat-shock proteins, ATP synthase, Carbohydrate metabolism, Transcription/translation, Detoxification enzymes, and 4 unidentified protein categories. Furthermore, expression pattern analysis of the expressed proteins under salt stress is an important step in identifying mechanisms of stress resistance in different plants including tomatoes.